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KMID : 0358219920190010001
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Korean Journal of Fertility and Sterility
1992 Volume.19 No. 1 p.1 ~ p.8
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The Use of FDA to Assess the Viability of Preimplantation Mouse Embryo In vitro
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김재명
홍진기/서병희/이재현/정길생
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Abstract
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A fluorescence microscopy technique using flurescein diacetate (FDA) as a substract has been tested for the evaluation of the viability of early mouse embryos. Embryos were incubated in T6 containing FDA concentrations of 2.5 to 50μg/ml for 1 to
5min.
Embryos were then examined by reflected light fluorescence using a KP 490 and 520 barrier filter in a Nicon Diaphot microscopy.
@ES the results were as follow.
@EN 1. The rate of fluorescein accumulation increased on the concentration on FDA from 2.5×10E-6M to 20×10E-6M
2. The rate at which intracellular fluorescein was lost from embryos was depended on the temperature at which are stored.
3. Embryos with 3 min exposure to FDA have the most intensity of fluorescence.
4. Exposure of 2 ce3ll embryos to FDA (2.5-5μg/ml) for 1 min did not alter their ability to delope normally in vitro.
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KEYWORD
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